Congrès annuel de la SFT - Brest, 13 et 14 octobre 2005
"Allergies et Toxiques " The protective effect of aluminosilicate clay against zearalenone toxicity in mice (a) Laboratory of Environmental Immunology and Microbiology, Faculty of Sciences Bizerte-7021-Zarzouna, Tunisia. * Corresponding author. Tel.: +216 72 59 18 45 poste 22 57 fax: +216 72 59 05 66. Hydrated sodium calcium aluminosilicate (HSCAS), an anticaking agent for mixed feed, was added alone or simultaneously with a toxic Zearalenone (ZEN) dose to balb/c mice and was evaluated for its ability to restore damages induced by ZEN. The latter is a mycotoxin produced by fusarium genera ; it is mainly known to induce several toxic effects on animals and humans; such as hepatotoxicity, immunotoxicity and nephrotoxicity. The experimental approach consisted of 7 treatments of 6 mice each by 50, 200, 400 mg/kg bw of HSCAS. Two experimented groups have received respectively ZEN alone (40 mg/kg representing 8% of LD 50 ) and ZEN with the highest dose of HSCAS (400 mg/kg bw). The control groups received water or olive oil. 48 hours after treatment, blood samples were collected for haematological and serum biochemical parameters measurements. ZEN treatment significantly increased hematocrit , haemoglobin, white blood cells: lymphocytes, eosinophils, neutrophils, monocytes and the most of biochemical serum parameters; it significantly reduced platelets and induced degenerative changes in the hepatic and renal tissues; while, the mixture of HSCAS with ZEN induced a reestablishment of haematological parameters, levels of serum biochemical enzyme activities and histological pictures of both liver and kidney. Thus, our data strongly suggested that deleterious effects of ZEN could be overcome or, at least, significantly diminished by HSCAS. Moreover, this sorbent by itself did not show any toxic effects.
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